Orange Alert

Syracuse University, College of Arts and Sciences

Instruments

Instruments and Associated Fees

All fees are hourly unless otherwise noted.

A BLS2 Biosafety Cabinet is available for users of the Blatt BioImaging Center $10/hour.

Zeiss LSM 980 with Airyscan 2 confocal microscope (inverted, fully motorized)

Zeiss LSM 980 with Airyscan2 confocal microscope in the Blatt Bioimaging Center.

Features include:

  • DIC, brightfield and fluorescence
  • Halogen lamp 12V 100W for transmitted light and Colibri 5 Type RGB UV for epifluorescence
  • Lasers: 405 nm, 488 nm, 561 nm and 639 nm
  • Detectors: 1 T-PMT, 1 GaAsP detector, 2 MA-PMT’s, Airyscan 2 Multiplex with 4Y and 8Y
  • Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Neofluar 40X/1.2 NA Imm Corr DIC, and a C Plan-Apochromat 63X/1.4 NA oil DIC
  • Zeiss Zen 3.3

Training takes 1-3 hours, depending on prior experience.

Zeiss LSM 980 Airyscan 2 rates and fees

Description Rate
Internal Base Rate $40/hour
Initial Training Fee $40/hour
Assisted Usage $50/hour
Off-Primetime (weekdays 6 p.m. - 9 a.m., weekends and holidays) $20/hour
Whole day $220/day
Primetime non SU Users, including SUNY-UMU users $45/hour
Off Primetime/weekends/holidays non SU users, including SUNY-UMU users $25/hour
Commerical Users $60/hour
Emergency Sample drop off and analysis $60/hour

Zeiss LSM 710 confocal microscope (inverted, fully motorized)

Zeiss LSM 710 confocal microscope in the Blatt Bioimaging Center.

Features include:

  • DIC, brightfield and fluorescence
  • Halogen lamp 12V 100W for transmitted light and X-Cite 120 XL for epifluorescence
  • Lasers: 405 nm diode laser, a multiline Argon (458/488/514 nm), and a HeNe (543 nm)
  • Detectors: 1 T-PMT, 1 GaAsP detector, 2 highly sensitive low dark-noise PMT’s
  • Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Apochromat 20X/0.8 NA, Plan-Neofluar 40X/1.3 NA oil DIC, and a Plan-Apochromat 63X/1.4 NA oil DIC
  • Zeiss Zen 3.1

Training takes 1-3 hours, depending on prior experience.

Zeiss LSM 710 rates

Description Rate
Internal Base Rate $30/hour
Training Fee $40/hour
Assisted Usage $50/hour
Off Primetime (weekdays 6 p.m. - 9 a.m., weekends, holidays) $10/hour
Whole Day $160/day

Leica DMi8 Confocal and Digital Lightsheet

microscope

Features include:

  • SP8 laser Scanning confocal module
  • Inverted, fully motorized
  • Brightfield and fluorescence
  • Incubation chamber and CO2 control.
  • Halogen lamp 12V 100W for transmitted light and EL6000 Mercury lamp for epifluorescence
  • Lasers: 405 nm, 488 nm, 561 nm and 639 nm
  • Detectors: 2 PMT SP, 2 HyD SP
  • Objective lenses: HC PL APO 10x/0.40 CS2, HC PL APO 20x/0.75 IMM CORR CS2 objective, HC PL APO 40x/1.10 W CORR CS2 0.65, and an HCX Plan Apochromat 63 3 /1.40-0.06 NA OIL
  • Software: LAS X SP8 Control Software
  • Training takes 1-2 hours depending on experience.

  • Digital Light Sheet module—assisted usage only:
  • Objectives: HC PL FLUOTAR 2.5x/0.07, HC PL FLUOTAR 5x/0.15, HC APO L10x/0.30 W DLS, HC FLUOTAR L25x/0.95 W DLS
  • Mirrors: DLS TwinFlect 2.5mm, DLS TwinFlect 5mm
  • Filters: Filter DLS 455-495, Filter DLS 504-545, Filter DLS 575-615, Filter DLS 405/488/552/638
  • Sample holders: U-Shaped Capillaries, 20mm x 2.5mm, U-Shaped Capillaries, 20mm x 1.5mm, FEP Tube 10 x 25 cm
  • Minimum session time is 3 hours.

Leica DMi8 Confocal and Digital Lightsheet

Description Rate
Internal/Training Rate $23/hour
Internal Assisted Usage Rate $50/hour
Non-SU Users Base/Training Rate $40/hour
Off-Primetime Internal users (9:00 am- 6:00 pm, weekends and holidays $16/hour
Off-Primetime non-SU users (9:00 am- 6:00 pm, weekends and holidays $23/hour
Commercial Users $60/hour
Commercial Users Assisted Rate $100/hour
Emergency Sample Drop off and Analysis $60/hour

Leica THUNDER- Upright Fluorescent Microscope

Microscope

THUNDER (T)— automated fluorescent compound microscope (Leica Thunder Imager Tissues) that allows for fast and easy 3D exploration of cells, tissues and organoids. Features include:

  • Upright, fully motorized
  • Brightfield, Polarized Light and fluorescence
  • Lumencor SOLA engine for Fluorescence illumination
  • Cameras: Leica DFC9000 GT sCMOS monochrome camera and DMC4500 CCD color camera
  • Objective lenses: HC PL APO 10x/0.45, HC FLUOTAR 16x/0.6 IMM CORR L VISIR, HC PL APO 20x/0.8, HC FLUOTAR 25x/0.95 W VISIR, HC APO 40x/0.8 W U-V-I, HC APO 63x/0.9, W U-V-I, HC PL APO 63x/1.40-0.60 OIL
  • Software: LAS X Control Software
  • THUNDER optical clearing algorithm

Training takes 30-2 hours depending on experience.

THUNDER_MO

THUNDER (MO)— automated fluorescent stereomicroscope (Leica Thunder Imager Model Organism) that allows for fast and easy 3D exploration of whole organisms. The systems are coupled to CellTram manual microinjectors for organism manipulation while imaging. Features include:

  • Automated fluorescent stereomicroscopes
  • Brightfield and fluorescence
  • Lumencor LED3 engine for Fluorescence illumination
  • Cameras: Leica DFC9000 GT sCMOS monochrome camera
  • Objective lenses:
  • Software: LAS X Control Software
  • THUNDER optical clearing algorithm

Training takes 30 min-2 hours depending on experience.

Leica THUNDER- Upright Fluorescent Microscope rates and fees

Description Rate
Internal/Training Rate $12/hour
Assisted Usage Rate $21/hour

Zeiss AxioObserver Z1 widefield microscope (inverted, fully motorized)

Zeiss AxioObserver Z1 widefield microscope in the Blatt Bioimaging Center.

Features include:

  • DIC, brightfield and fluorescence
  • Halogen lamp 12V 100W for transmitted light and PhotoFluor LM-75 for reflected light
  • Objective lenses: Plan-Neofluar 40X/1.3 NA oil DIC; Plan-Apochromat 100X/1.4 NA oil DIC
  • Fluorescence filter sets: DAPI, FITC, Cy3, CFP and YFP
  • Hamamatsu CMOS Orca Flash 4.0 LT camera
  • MetaMorph acquisition and image analysis software

Training takes 1-2 hours, depending on prior experience.

Zeiss Axio Observer Z1 Widefield rates and fees

Description Rate
Internal Base Rate $15/hour
Training Fee $20/hour
Assisted Usage $20/hour
Off Primetime (weekdays 6 p.m. - 9 a.m., weekends, holidays) $15/hour