Instruments and Associated Fees

• Dragonfly-620 SR spinning disk confocal
• Zeiss LSM 980 with Airyscan 2 confocal microscope
• Biovision spinning disk confocal microscope
• Leica DMi8 Confocal and Digital Lightsheet
• Leica THUNDER- Upright Fluorescent Microscope
• Leica Fluorescent Stereoscope
• Imaris - Microscopy Image Analysis Software

All fees are hourly unless otherwise noted.

A BLS2 Biosafety Cabinet is available for users of the Blatt BioImaging Center $10/hour.

Instrument reservations

Training

Dragonfly-620 SR spinning disk confocal

Features include:

• Widefield and fluorescence
• Spinning disk confocal, and TIRF single molecule imaging microscope paired with a Leica DMi8 and High-Powered Laser Engine.
• The following excitation lines will be available: 405, 488, 560, 640, and 730.
• A Mosaic3 DMD for photostimulation experiments such as FRAP and photoactivation and conversion.
• MicroPoint Gen 4 for ablation, uncaging, FRAP, photo-conversion, activation, and optogenetics.
• The following Leica objectives are available with the system: 20x air, 25x water immersion, 40x water immersion, 40x oil, 63x glycerol, and 100x TIRF.
• An Okolab stage-top incubation kit for environmental control of temperature, CO2, and humidity.
• Dual Camera imaging: ZL41 sCMOS and iXON EMCCD
  
  

Dragonfly Spinning disk confocal rates and fees

Zeiss LSM 980 with Airyscan 2 confocal microscope (inverted, fully motorized)

Features include:

• DIC, brightfield and fluorescence
• Halogen lamp 12V 100W for transmitted light and Colibri 5 Type RGB UV for epifluorescence
• Lasers: 405 nm, 488 nm, 561 nm and 639 nm
• Detectors: 1 T-PMT, 1 GaAsP detector, 2 MA-PMT’s, Airyscan 2 Multiplex with 4Y and 8Y
• Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Neofluar 40X/1.2 NA Imm Corr DIC, and a C Plan-Apochromat 63X/1.4 NA oil DIC
• Zeiss Zen 3.3

Training takes 1-3 hours, depending on prior experience.

Zeiss LSM 980 Airyscan 2 rates and fees

Biovision spinning disk confocal microscope

Features include:

• X-Light V3 Spinning Disk Confocal System
• DIC, Widefield and fluorescence
• CELESTA light engine - 5 independently controlled laser light sources with 405nm(400mW), 488nm(700mW), 545nm (700mW), 589mm(200mW), 639nm(700mW)
• Available objectives: 20x air, 40x oil and 63x oil.
• Camera: PCO Edge sCMOS
• Software: Visiview Silver Imaging and analyses software

Biovision spinning disk confocal microscope

Leica DMi8 Confocal and Digital Lightsheet

Features include:

• SP8 laser Scanning confocal module
• Inverted, fully motorized
• Brightfield and fluorescence
• Incubation chamber and CO2 control.
• Halogen lamp 12V 100W for transmitted light and EL6000 Mercury lamp for epifluorescence
• Lasers: 405 nm, 488 nm, 561 nm and 639 nm
• Detectors: 2 PMT SP, 2 HyD SP
• Objective lenses: HC PL APO 10x/0.40 CS2, HC PL APO 20x/0.75 IMM CORR CS2 objective, HC PL APO 40x/1.10 W CORR CS2 0.65, and an HCX Plan Apochromat 63 3 /1.40-0.06 NA OIL
• Software: LAS X SP8 Control Software
• Training takes 1-2 hours depending on experience.

• Digital Light Sheet module—assisted usage only:
• Objectives: HC PL FLUOTAR 2.5x/0.07, HC PL FLUOTAR 5x/0.15, HC APO L10x/0.30 W DLS, HC FLUOTAR L25x/0.95 W DLS
• Mirrors: DLS TwinFlect 2.5mm, DLS TwinFlect 5mm
• Filters: Filter DLS 455-495, Filter DLS 504-545, Filter DLS 575-615, Filter DLS 405/488/552/638
• Sample holders: U-Shaped Capillaries, 20mm x 2.5mm, U-Shaped Capillaries, 20mm x 1.5mm, FEP Tube 10 x 25 cm
• Minimum session time is 3 hours.

Leica DMi8 Confocal and Digital Lightsheet

Leica THUNDER- Upright Fluorescent Microscope

THUNDER (T)— automated fluorescent compound microscope (Leica Thunder Imager Tissues) that allows for fast and easy 3D exploration of cells, tissues and organoids. Features include:

• Upright, fully motorized
• Brightfield, Polarized Light and fluorescence
• Lumencor SOLA engine for Fluorescence illumination
• Cameras: Leica DFC9000 GT sCMOS monochrome camera and DMC4500 CCD color camera
• Objective lenses: HC PL APO 10x/0.45, HC FLUOTAR 16x/0.6 IMM CORR L VISIR, HC PL APO 20x/0.8, HC FLUOTAR 25x/0.95 W VISIR, HC APO 40x/0.8 W U-V-I, HC APO 63x/0.9, W U-V-I, HC PL APO 63x/1.40-0.60 OIL
• Software: LAS X Control Software
• THUNDER optical clearing algorithm

Training takes 30-2 hours depending on experience.

Leica THUNDER- Upright Fluorescent Microscope

Leica Fluorescent Stereoscope

Leica Fluorescent Stereoscope— automated fluorescent stereomicroscope (Leica Thunder Imager Model Organism) that allows for fast and easy 3D exploration of whole organisms. The systems are coupled to CellTram manual microinjectors for organism manipulation while imaging. Features include:

• Automated fluorescent stereomicroscopes
• Brightfield and fluorescence
• Lumencor LED3 engine for Fluorescence illumination
• Cameras: Leica DFC9000 GT sCMOS monochrome camera
• Objective lenses:
• Software: LAS X Control Software
• THUNDER optical clearing algorithm

Training takes 30 min-2 hours depending on experience.

Leica Fluorescent Stereoscope rates and fees

Imaris - Microscopy Image Analysis Software

Imaris - Microscopy Image Analysis Software

Imaris is an advanced 3D and 4D image visualization and analysis platform designed for microscopy datasets. Software includes powerful tools for surface rendering, spot detection, tracking, and colocalization analysis, Imaris facilitates the extraction of meaningful quantitative data from large multidimensional datasets.

Imaris - Microscopy Image Analysis Software rates and fees