Instruments
Instruments and Associated Fees
- Zeiss LSM 980 with Airyscan 2 confocal microscope
- Zeiss LSM 710 confocal microscope
- Leica DMi8 Confocal and Digital Lightsheet
- Leica THUNDER Tissue- Upright Fluorescent Microscope
- Zeiss AxioObserver Z1 widefield microscope
All fees are hourly unless otherwise noted.
A BLS2 Biosafety Cabinet is available for users of the Blatt BioImaging Center $10/hour.
Zeiss LSM 980 with Airyscan 2 confocal microscope (inverted, fully motorized)
Features include:
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and Colibri 5 Type RGB UV for epifluorescence
- Lasers: 405 nm, 488 nm, 561 nm and 639 nm
- Detectors: 1 T-PMT, 1 GaAsP detector, 2 MA-PMT’s, Airyscan 2 Multiplex with 4Y and 8Y
- Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Neofluar 40X/1.2 NA Imm Corr DIC, and a C Plan-Apochromat 63X/1.4 NA oil DIC
- Zeiss Zen 3.3
Training takes 1-3 hours, depending on prior experience.
Zeiss LSM 980 Airyscan 2 rates and fees
| Description | Rate |
|---|---|
| Internal Base Rate | $40/hour |
| Initial Training Fee | $40/hour |
| Assisted Usage | $50/hour |
| Off-Primetime (weekdays 6 p.m. - 9 a.m., weekends and holidays) | $20/hour |
| Whole day | $220/day |
| Primetime non SU Users, including SUNY-UMU users | $45/hour |
| Off Primetime/weekends/holidays non SU users, including SUNY-UMU users | $25/hour |
| Commerical Users | $60/hour |
| Emergency Sample drop off and analysis | $60/hour |
Zeiss LSM 710 confocal microscope (inverted, fully motorized)
Features include:
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and X-Cite 120 XL for epifluorescence
- Lasers: 405 nm diode laser, a multiline Argon (458/488/514 nm), and a HeNe (543 nm)
- Detectors: 1 T-PMT, 1 GaAsP detector, 2 highly sensitive low dark-noise PMT’s
- Objective lenses: Plan-Neofluar 10X/0.3 NA, Plan-Apochromat 20X/0.8 NA, Plan-Neofluar 40X/1.3 NA oil DIC, and a Plan-Apochromat 63X/1.4 NA oil DIC
- Zeiss Zen 3.1
Training takes 1-3 hours, depending on prior experience.
Zeiss LSM 710 rates
| Description | Rate |
|---|---|
| Internal Base Rate | $30/hour |
| Training Fee | $40/hour |
| Assisted Usage | $50/hour |
| Off Primetime (weekdays 6 p.m. - 9 a.m., weekends, holidays) | $10/hour |
| Whole Day | $160/day |
Leica DMi8 Confocal and Digital Lightsheet
Features include:
- SP8 laser Scanning confocal module
- Inverted, fully motorized
- Brightfield and fluorescence
- Incubation chamber and CO2 control.
- Halogen lamp 12V 100W for transmitted light and EL6000 Mercury lamp for epifluorescence
- Lasers: 405 nm, 488 nm, 561 nm and 639 nm
- Detectors: 2 PMT SP, 2 HyD SP
- Objective lenses: HC PL APO 10x/0.40 CS2, HC PL APO 20x/0.75 IMM CORR CS2 objective, HC PL APO 40x/1.10 W CORR CS2 0.65, and an HCX Plan Apochromat 63 3 /1.40-0.06 NA OIL
- Software: LAS X SP8 Control Software
- Training takes 1-2 hours depending on experience.
- Digital Light Sheet module—assisted usage only:
- Objectives: HC PL FLUOTAR 2.5x/0.07, HC PL FLUOTAR 5x/0.15, HC APO L10x/0.30 W DLS, HC FLUOTAR L25x/0.95 W DLS
- Mirrors: DLS TwinFlect 2.5mm, DLS TwinFlect 5mm
- Filters: Filter DLS 455-495, Filter DLS 504-545, Filter DLS 575-615, Filter DLS 405/488/552/638
- Sample holders: U-Shaped Capillaries, 20mm x 2.5mm, U-Shaped Capillaries, 20mm x 1.5mm, FEP Tube 10 x 25 cm
- Minimum session time is 3 hours.
Leica DMi8 Confocal and Digital Lightsheet
| Description | Rate |
|---|---|
| Internal/Training Rate | $23/hour |
| Internal Assisted Usage Rate | $50/hour |
| Non-SU Users Base/Training Rate | $40/hour |
| Off-Primetime Internal users (9:00 am- 6:00 pm, weekends and holidays | $16/hour |
| Off-Primetime non-SU users (9:00 am- 6:00 pm, weekends and holidays | $23/hour |
| Commercial Users | $60/hour |
| Commercial Users Assisted Rate | $100/hour |
| Emergency Sample Drop off and Analysis | $60/hour |
Leica THUNDER Tissue- Upright Fluorescent Microscope
Features include:
- Upright, fully motorized
- Brightfield, Polarized Light and fluorescence
- Lumencor SOLA engine for Fluorescence illumination
- Camers: Leica DFC9000 GT sCMOS monochrome camera and DMC4500 CCD color camera
- Objective lenses: HC PL APO 10x/0.45, HC FLUOTAR 16x/0.6 IMM CORR L VISIR, HC PL APO 20x/0.8, HC FLUOTAR 25x/0.95 W VISIR, HC APO 40x/0.8 W U-V-I, HC APO 63x/0.9, W U-V-I, HC PL APO 63x/1.40-0.60 OIL
- Software: LAS X Control Software
- Training takes 30-2 hours depending on experience.
Leica THUNDER Tissue- Upright Fluorescent Microscope rates and fees
| Description | Rate |
|---|---|
| Internal/Training Rate | $12/hour |
| Assisted Usage Rate | $21/hour |
Zeiss AxioObserver Z1 widefield microscope (inverted, fully motorized)
Features include:
- DIC, brightfield and fluorescence
- Halogen lamp 12V 100W for transmitted light and PhotoFluor LM-75 for reflected light
- Objective lenses: Plan-Neofluar 40X/1.3 NA oil DIC; Plan-Apochromat 100X/1.4 NA oil DIC
- Fluorescence filter sets: DAPI, FITC, Cy3, CFP and YFP
- Hamamatsu CMOS Orca Flash 4.0 LT camera
- MetaMorph acquisition and image analysis software
Training takes 1-2 hours, depending on prior experience.
Zeiss Axio Observer Z1 Widefield rates and fees
| Description | Rate |
|---|---|
| Internal Base Rate | $15/hour |
| Training Fee | $20/hour |
| Assisted Usage | $20/hour |
| Off Primetime (weekdays 6 p.m. - 9 a.m., weekends, holidays) | $15/hour |